About Pluto

Pluto is a chemically defined, serum-free, xeno-free medium for human pluripotent stem cells (hPSC) culture.
hPSC remain its pluripotency after long-term culture using Pluto.

Storage : -15°C or below, protect from light.
Shelf Life : 12 months
Avoid freeze / thaw cycles

Product Name Catalog Number Size Grade
Pluto ES medium RL001 500 mL Research Use Only
Pluto ES supplement RL005S 10mL Research Use Only


Embryonic stem cells (ESC) are the inner cell mass from blastocyst. When culturing ex-vivo, these PSC can grow, replicate and retain the ability to differentiate. These features have made PSC the focus of research. Especially after the induced pluripotent stem cell (iPSC) technique was developed by Professor Yamanaka and followed by the Nobel Prize in Physiology or Medicine in 2012. The investment in hPSC research is even more tremendous.

But hPSC culture ex-vivo is rather complicated. Traditionally, a layer of “feeder cells” must be cultured prior to hPSC, and the hPSC can then be cultured on the feeder cells. The results can easily be affected by operational variations, the sources of feeder cells and cell-cell interactions etc.

To be grateful to some of the big names in biochemical area, feeder cells are no longer required in hPSC culture. When pre-coating with certain extracellular matrix (ECM), hPSC can be cultured in-vitro without feeder cells.

  • Pluto, which is a chemically defined medium, can support hPSC growth without feeder cells. The chemically defined formulation can reduce the variations and make hPSC culture easier than it used to be.
  • hPSC remain undifferentiated and retain its pluripotency after long-term culture using Pluto. With specific stimulations, these hPSCs could differentiate to three germ layer cells.
  • Pluto is manufactured under GMP and ISO 13485 certified factory, and the culture performance is tested from batch to batch.


Cell morphology of hESC cultured using Pluto


H9 (hESC) cultured in Pluto human iPS/ES cell culture medium on Geltrex-coated plates display a colony morphology typical of normal feeder free iPS/ES cell cultures, including high nucleus-to-cytoplasm ratio, prominent nucleoli, and distinct colony edges.

Compare Pluto to commercial hPSC medium


The equivalent study demonstrates Pluto human iPS/ES cell culture medium has the same performance of proliferation with commercial product.

hPSC cultured using Pluto are pluripotency markers positive


Flow cytometry results show that the pluripotency maintenance is similar in Pluto human iPS/ES cell culture medium and commercial media products in feeder and feeder-free culture systems.

The differentiation potential remains after hESC cultured using Pluto


H9 cultured using Pluto can be induced and differentiate into : (A) Three germ layers; (B) Teratomas.
Arrow: Gland; Arrow head: Bone; Star: Melanocyte


User manual and more information are available upon request.

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