DuoTSM Supplement

About DuoTSM

DuoTSM is a chemically defined supplement for human ADSC and UC-MSC culture.

 • Mixed with 500mL of recommended basal medium before use
 • Store at -15 ℃ or below. Protect from light
 •  Avoid freeze / thaw cycles
 • Do not warm DuoTSM in a water bath above 28℃

Product Name Catalog Number Size
DuoTSM Supplement  MED009 10 mL

Overview

Mesenchymal Stem Cells (MSCs) have demonstrated great potential in therapeutic / regenerative application.

However, from the laboratory to the clinic isn’t an easy path, as there are regulations on safety and verification.
Also it’s rather difficult to maintain the quality and the consistency of the MSC especially during scale up.

In order to gain control over culture conditions, the chemically defined culture medium is a priority choice to most cell biologists, and the traditional FBS culture is disappearing.

DuoTSM is our first chemically defined MSC culture supplement, and it features:

  1. MSC can attach and grow on cell culture plates efficiently without pre-coated ECM.
  2. Minimum risk from human / animal contamination.
  3. Advanced consistency and improved scalability.
  4. Defined formula has great potential for collecting and studying exosomes.
  5. Excellent MSC culture performance.
  6. MSC remain their stemness (like the ability to differentiate) after few passages cultured in DuoTSM.

Data

Doubling time of ADSC and WJ-MSC cultured with DuoTSM or 5% hPL.

ADSC or WJ-MSC cultured with DuoTSM or 5% hPL. Cells cultured with DuoTSM demonstrate competitive doubling time.

Long-term culture of ADSC and WJ-MSC were performed with DuoTSM

DuoTSM-2#2

ADSC or WJ-MSC cultured with DuoTSM demonstrate the ability for long-term culture.

ADSC and WJ-MSC remain typical morphology cultured with DuoTSM

DuoTSM-3

MSC adhesion assessment with DuoTSM

DuoTSM-4

 

  • Seeding density: 10,000 cells/cm²
  • Culture plate: Falcon TC-treated 24 well (353047), without coating.
  • Cells were washed twice with PBS at 20, 40 and 60 minutes after seeding, and then fixed.
  • 1% crystal violet stain was performed, pictures were taken and analysis with the diagram on the left.

MSC cultured in DuoTSM express typical MSC markers

DuoTSM-6

Flow cytometry was performed with MSC cultured 5 passages in DuoTSM.

Differentiation ability of MSC cultured in DuoTSM

After cultured with DuoTSM, MSC demonstrates the ability to differentiate into osteocyte, adipocyte and chondrocyte.

Documents

Flyer download
User manual and more information are available upon request.

info@duogenicsc.com

if you have any questions,
feel free to contact us.

We are glad to provide you with further information.