The potential and application of hPSC have always been the focus of research in biomedical area. Especially after the induced pluripotent stem cell (iPSC) technique was developed by Professor Yamanaka and followed by the Nobel Prize in Physiology or Medicine in 2012. The investment in hPSC research is even more tremendous.

But hPSC culture ex-vivo is rather complicated. Traditionally, a layer of “feeder cells” must be cultured prior to hPSC, and the hPSC can then be cultured on the feeder cells. The results can easily be affected by operational variations and cell-cell interactions.

Thanks to some of the big names in biochemical area, feeder cells are no longer required in hPSC culture. When pre-coating with certain extracellular matrix (ECM), hPSC can be cultured in-vitro without feeder cells. This makes hPSC culture easier than it used to be.

And now we have developed the next generation hPSC culture medium: DuoESy. Not only free from feeder cells but also free from pre-coating procedure. With our special formulation, the inconvenience of awaiting time and selecting pre-coating material is over.

DuoESy is a xeno free medium designed for hPSCs culture, manufactured under GMP and ISO 13485 certified factory. Using DuoESy to culture hPSC doesn’t require any other supplement, such as ROCK inhibitor or growth factors. This will also minimize human error.

With the weekend free culture option, DuoESy medium provide a better flexibility for your workflow. hPSCs remain undifferentiated and expand normally over 10 passages in DuoESy medium. With specific stimulations, these hPSCs could differentiate to three germ layer cells.

With the breakthrough of our DuoESy medium, hPSC culture and scale up become a lot easier.