Chemically defined · Serum-free · Xeno-free medium for hPSC culture
Pluto is a chemically defined, serum-free, xeno-free medium for human pluripotent stem cells (hPSC) culture. hPSC remain their pluripotency after long-term culture using Pluto.
| Product Name | Catalog Number | Size | Grade |
|---|---|---|---|
| Pluto ES medium | RL001 | 500 mL / bottle | Research Use Only |
| Pluto ES supplement | RL005S | 10 mL / bottle | Research Use Only |
Embryonic stem cells (ESC) are the inner cell mass from blastocyst. When culturing ex-vivo, these PSC can grow, replicate and retain the ability to differentiate. These features have made PSC the focus of research. Especially after the induced pluripotent stem cell (iPSC) technique was developed by Professor Yamanaka and followed by the Nobel Prize in Physiology or Medicine in 2012, the investment in hPSC research has been even more tremendous.
hPSC culture ex-vivo is rather complicated. Traditionally, a layer of "feeder cells" must be cultured prior to hPSC, and the hPSC can then be cultured on the feeder cells. The results can easily be affected by operational variations, the sources of feeder cells and cell–cell interactions, etc.
Thanks to some of the big names in biochemical area, feeder cells are no longer required in hPSC culture. When pre-coating with certain extracellular matrix (ECM), hPSC can be cultured in vitro without feeder cells.
Pluto, a chemically defined medium, can support hPSC growth without feeder cells. The chemically defined formulation reduces variations and makes hPSC culture easier than it used to be.
hPSC remain undifferentiated and retain their pluripotency after long-term culture using Pluto. With specific stimulations, these hPSCs can differentiate into three germ layer cells.
Pluto is manufactured under GMP and ISO 13485 certified facility, with culture performance tested batch-to-batch.
H9 (hESC) cultured in Pluto human iPS/ES cell culture medium on Geltrex-coated plates display a colony morphology typical of normal feeder-free iPS/ES cell cultures, including high nucleus-to-cytoplasm ratio, prominent nucleoli, and distinct colony edges.
The equivalent study demonstrates Pluto human iPS/ES cell culture medium has the same proliferation performance as commercial products.
Flow cytometry results show that the pluripotency maintenance is similar in Pluto human iPS/ES cell culture medium and commercial media products in feeder and feeder-free culture systems.
Ectoderm
Mesoderm
Endoderm
H9 cultured using Pluto can be induced and differentiate into: (A) Three germ layers; (B) Teratomas. Arrow: Gland; Arrow head: Bone; Star: Melanocyte.
All culture media products are available for CMO (Contract Manufacturing) and CDMO (Contract Development and Manufacturing) services, including formula customisation, specification tailoring, regulatory documentation, and full-scale support from small batches to mass production.
User manual and more information are available upon request.