3D Cell Culture System

Closed Cell Culture System
Bioreactor & Closed System

A large-scale cell expansion solution combining a closed gas-permeable culture bag, specialized microcarriers, and AllPhase medium.

System Overview

Our Closed Cell Culture System consists of a closed, gas-permeable culture bag, specialized microcarriers, and AllPhase High-Performance MSC Proliferation Medium. By utilizing a programmable Cell Rocker for intermittent agitation, the system minimizes cell aggregation, a common challenge in large-scale cell culture. Following culture completion, our streamlined cell harvesting protocol enables rapid and simple recovery of the expanded cells.

Key Features

01Minimal equipment requirements
02User-friendly operation
03Fully closed system

System Workflow Diagram

Closed Cell Culture System workflow diagram
Efficacy We Achieved

With our Closed rocking bag culture system, human adipose-derived stem cells (hADSCs) achieved a 550-fold expansion within 18 days. Post-expansion, the cells successfully retained their MSC characteristics, including specific cell surface marker expression and tri-lineage differentiation potential.

Tri-lineage differentiation: Osteogenesis, Chondrogenesis, Adipogenesis
Flow Cytometry surface markers: CD44 / CD90 / CD73 / CD105 (Flask vs PVA carrier)

MSC negative markers cocktail(CD34, CD11b, CD19, CD45, HLA-DR)