DuoVEC

DuoVEC – Supplement

Xeno-free supplement for human endothelial cell culture

About DuoVEC

DuoVEC Supplement for hEC

DuoVEC is a xeno-free supplement optimized for human endothelial cells (hEC) culture. Human umbilical vein endothelial cells (HUVEC) cultured in DuoVEC express typical EC protein markers throughout P1 to P7, with cell doubling time < 40 hours.

Supporting proliferation of primary vascular endothelial cells
Xeno-free
GMP-compliant manufacturing

Specifications

Storage

−15°C or below, protect from light

Shelf Life

12 months

Notes

Avoid freeze / thaw cycles

Product Name	Catalog Number	Size	Grade
DuoVEC Supplement	DVM500-S	10 mL / bottle	Research Use Only

Overview

Research in endothelial cells has long been a hotspot in biomedical area: cardiovascular disease is the leading cause of death worldwide; angiogenesis plays a critical part in tumor formation; and there is a global shortage of corneal donation.

ECs take part in blood pressure regulation, arteriosclerosis, angiogenesis, coagulation and so on. HUVEC provides a classic model system to study many aspects of endothelial function and disease, including cardiovascular and metabolic diseases. This model is still widely used today mainly because of the high HUVEC isolation success rate.

DuoVEC is a supplement optimized for hEC culture; the xeno-free formula bridges research use to clinical use. HUVEC cultured in medium with FBS can be transferred to the DuoVEC culture system.

DuoVEC is manufactured under GMP and ISO 13485 certified facility, with culture performance tested batch-to-batch.

Data

Cell morphology of HUVEC primary culture in DuoVEC

100 ×

200 ×

The umbilical cord surface was washed with IMDM (or PBS), and the same medium was injected inside the umbilical vein by syringe to remove the clots. IMDM with 2 mg/ml collagenase NB 4 (SERVA) was then injected into the umbilical vein and left for 30 minutes at room temperature. The medium was then squeezed out from the umbilical cord and centrifuged at 15,000 rpm for 5 minutes. The cells were seeded on 100 mm TC treated culture plates pre-coated with 10 µg/ml fibronectin. The pictures were taken 5 days after isolation.

Angiogenesis potential of HUVEC culturing using DuoVEC

HUVEC were isolated and cultured for 1 passage prior to this experiment. Cells were then seeded on a 96-well culture plate pre-coated with 100 µl Matrigel. Cells were left in a 37°C, 5% CO2 incubator for at least 4 hours before the pictures were taken.

HUVEC culturing in DuoVEC exhibit competitive growth rate to medium with FBS

Doubling time of HUVEC (from P4 to P6) culturing in:
1. DuoVEC
2. Brand B XF medium
3. Brand P FBS medium
4. Brand L FBS medium

HUVEC grow better in DuoVEC than in Brand B Xeno-Free medium. DuoVEC provides a competitive growth rate when culturing HUVEC compared to FBS medium.

HUVEC express typical endothelial markers when culturing in DuoVEC

Flow cytometry analysis of HUVEC cultured in DuoVEC at P1 and P7. CD31 and CD144 are both highly expressed.

Custom Services

CMO / CDMO Custom Manufacturing Services

All culture media products are available for CMO (Contract Manufacturing) and CDMO (Contract Development and Manufacturing) services, including formula customisation, specification tailoring, regulatory documentation, and full-scale support from small batches to mass production.

Documents

User manual and more information are available upon request.