DuoTSM

DuoTSM Supplement

Chemically defined supplement for human ADSC and UC-MSC culture

DuoTSM
About DuoTSM

DuoTSM Chemically Defined MSC Supplement

DuoTSM is a chemically defined supplement for human ADSC and UC-MSC culture.

  • Mixed with 500 mL of recommended basal medium before use
  • Store at −15°C or below, protect from light
  • Avoid freeze / thaw cycles
  • Do not warm DuoTSM in a water bath above 28°C
Specifications

Specifications

Storage
−15°C or below, protect from light
Usage
Mix with 500 mL of recommended basal medium before use
Notes
Avoid freeze / thaw cycles; do not warm above 28°C
Product NameCatalog NumberSizeGrade
DuoTSM SupplementMED00910 mL / bottleResearch Use Only
Overview

Overview

Mesenchymal Stem Cells (MSCs) have demonstrated great potential in therapeutic / regenerative application.

However from the laboratory to the clinic isn't an easy path, as there are regulations on safety and verification. Also it's rather difficult to maintain the quality and the consistency of the MSC especially during scale up.

In order to gain control over culture conditions, the chemically defined culture medium is a priority choice to most cell biologists, and the traditional FBS culture is disappearing.

DuoTSM is our first chemically defined MSC culture supplement, and it features:

  1. MSC can attach and grow on cell culture plates efficiently without pre-coated ECM
  2. Minimum risk from human / animal contamination
  3. Advanced consistency and improved scalability
  4. Defined formula has great potential for collecting and studying exosomes
  5. Excellent MSC culture performance
  6. MSC remain their stemness (like the ability to differentiate) after few passages cultured in DuoTSM
Data

Data

Doubling time of ADSC and WJ-MSC cultured with DuoTSM or 5% hPL

ADSC doubling time

ADSC

WJ-MSC doubling time

WJ-MSC

DuoTSM
5% hPL

ADSC or WJ-MSC cultured with DuoTSM or 5% hPL. Cells cultured with DuoTSM demonstrate competitive doubling time.

Long-term culture of ADSC and WJ-MSC were performed with DuoTSM

ADSC long-term culture P2–P7

ADSC

WJ-MSC long-term culture P4–P9

WJ-MSC

ADSC or WJ-MSC cultured with DuoTSM demonstrate the ability for long-term culture.

ADSC and WJ-MSC remain typical morphology cultured with DuoTSM

ADSC P3 Day3 morphology

ADSC, P3, Day3

WJ-MSC P5 Day3 morphology

WJ-MSC, P5, Day3

MSC adhesion assessment with DuoTSM

MSC adhesion 20 min

20 minutes

MSC adhesion 40 min

40 minutes

MSC adhesion 60 min

60 minutes

Area Index Chart

Area Index (20 / 40 / 60 min)

  • •Seeding density: 10,000 cells/cm²
  • •Culture plate: Falcon TC-treated 24-well (353047), without coating
  • •Cells were washed twice with PBS at 20, 40 and 60 minutes after seeding, and then fixed
  • •1% crystal violet stain was performed, pictures were taken and analysed with ImageJ

MSC cultured in DuoTSM express typical MSC markers

MSC markers CD90 CD73 CD44 CD105 negative

Flow cytometry was performed with MSC cultured 5 passages in DuoTSM.

Differentiation ability of MSC cultured in DuoTSM

Osteogenesis
Adipogenesis
Chondrogenesis
Differentiated
Differentiated Osteogenesis
Differentiated Adipogenesis
Differentiated Chondrogenesis
Control
Control Osteogenesis
Control Adipogenesis
Control Chondrogenesis

After cultured with DuoTSM, MSC demonstrates the ability to differentiate into osteocyte, adipocyte and chondrocyte.

Custom Services

CMO / CDMO Custom Manufacturing Services

All culture media products are available for CMO (Contract Manufacturing) and CDMO (Contract Development and Manufacturing) services, including formula customisation, specification tailoring, regulatory documentation, and full-scale support from small batches to mass production.

Documents

User manual and more information are available upon request.