Phenol red-free · Ultra-low background · Designed for EV research
| Product Name | Catalog Number | Size | Grade |
|---|---|---|---|
| DuoEXO – Supplement 50X | RUO003-S | 10 mL | Research Use Only |
Extracellular Vesicles (EVs) have gained importance since the early 2010s. MSC-derived EVs have been shown to be crucial in wound healing, angiogenesis and immune signaling. However, collection and purification are difficult — undesired exosomes are abundant in most MSC culture media.
DuoEXO is an EV-reduced, xeno-free cell culture supplement for hMSC. Despite the low background noise, MSCs secrete EVs and remain healthy for passages. DuoEXO makes it easier to collect and study MSC-derived EVs with less impurity.
Manufactured under GMP and ISO 13485 certified factory; culture performance is tested batch to batch.
Numbers of nano-particles (diameter 50–200 nm) in basal medium with:
The nano-particles in DuoEXO is 100 times smaller than other medium.
Nano-particle counts (50–200 nm) in DuoEXO are roughly 100× lower than basal medium with 10% FBS, exosome-depleted FBS, ultracentrifuged FBS, serum-free brand-G medium, or 5% hPL.
Doubling time of human umbilical cord-derived MSC (UC-MSC) cultured using either DuoEXO, 10% FBS or 5% hPL as supplement from P1 to P5.
Doubling time of UC-MSC from P1 to P5 cultured in DuoEXO is comparable to 10% FBS or 5% hPL.
Medium only
After MSCs cultured
CD9 (exosome marker) Western Blot
Comparison of 5% hPL and DuoEXO: the left panel was medium only, the right panel was medium after MSC culture. Medium was collected and followed by ultracentrifugation and Western Blot. (CD9: exosome marker) The result demonstrates that before culture, unidentified exosomes were detected in 5% hPL but not in DuoEXO. Exosomes were detected in DuoEXO after cultured with MSCs.
All culture media products are available for CMO (Contract Manufacturing) and CDMO (Contract Development and Manufacturing) services, including formula customisation, specification tailoring, regulatory documentation, and full-scale support from small batches to mass production.
User manual and more information are available upon request.