Xeno-free · Pre-coating-free · Weekend-free hPSC culture
*DuoESy-α and DuoESy-β are not to be sold separately
| Product Name | Catalog Number | Size | Grade |
|---|---|---|---|
| DuoESy (including DuoESy-α and DuoESy-β) | ERL001 | 100 + 400 mL | Research Use Only |
| DuoESy-α | ERL001-α | 100 mL | Research Use Only |
| DuoESy-β | ERL001-β | 400 mL | Research Use Only |
The potential and applications of hPSC have always been the focus of biomedical research — even more so after the iPSC technique by Prof. Yamanaka and the 2012 Nobel Prize in Physiology or Medicine.
hPSC culture ex-vivo is complicated. Traditionally, a layer of feeder cells must be cultured prior to hPSC, with results easily affected by operational variations and cell-cell interactions.
Feeder cells are no longer required when pre-coating with ECM — but DuoESy goes further: free from feeder cells AND free from pre-coating procedures.
No additional supplements (ROCK inhibitor, growth factors) needed, minimizing human error. With weekend-free option, DuoESy provides better workflow flexibility. hPSCs remain undifferentiated and expand normally over 10 passages, and can differentiate into three germ layer cells with specific stimulation.
H9 (hESC) or 201B7 (hiPSC) cultured using (A) conventional medium on ECM pre-coated plate; (B) conventional medium without ECM; (C) DuoESy without ECM.
H9 (hESC)
201B7 (hiPSC)
(A) Conventional medium on ECM pre-coated plate; (B) Conventional medium without ECM; (C) DuoESy without ECM
Day 1
Day 2
Day 3
hPSC cultured using DuoESy on TPP, Falcon or Corning plates without ECM pre-coating. Photos from day 1 to 3.
H9 (hESC) cultured using DuoESy without ECM exhibits similar doubling time to conventional medium with ECM pre-coating.
Oct-4 / Sox-2 / Nanog / c-Myc
SSEA4 / Tra-1-60
hPSC express the pluripotency markers Oct-4, Sox-2 Nanog and c-Myc after cultured using DuoESy for more than 10 passages
Neural cells
Tuj-1 / Nestin / DAPI
Endodermal cells
Sox17 / Sox2 / DAPI
Cardiac cells
TNNT2 / ACTN2 / DAPI
hPSC cultured in DuoESy retain ability to differentiate into endoderm, mesoderm and ectoderm cells.
All culture media products are available for CMO (Contract Manufacturing) and CDMO (Contract Development and Manufacturing) services, including formula customisation, specification tailoring, regulatory documentation, and full-scale support from small batches to mass production.
User manual, publications and more information are available upon request. Reference: Coating-Free Culture Medium for Establishing and Maintaining Human Induced Pluripotent Stem Cells, Cell Transplantation 2023.