AllPhase

AllPhase – Supplement

Clinical-grade · Xeno-free · Optimized for human MSC culture

AllPhase
About AllPhase

AllPhase Clinical-grade MSC Supplement

  • Clinical-grade MSC supplement
  • US FDA Master File Number registered
  • Approved under Taiwan Special Medical Techniques
  • Partnered in US IND trials
  • Made in USA
  • Superior primary culture efficiency
  • Xeno-free, no coating required
  • Doubling time < 30 hours
  • Suitable for clinical applications
Specifications

Specifications

Storage
-15°C or below, protect from light
Shelf Life
24 months
Notes
  • · Avoid freeze / thaw cycles
Product NameCatalog NumberSizeGrade
AllPhase – Supplement 20XAGA 1000-S50 mLClinical Grade
AllPhase – Supplement 50XAML 500-S10 mLResearch Use Only
Overview

Overview

AllPhase is a clinical-grade (approved in Regulations of Special Medical Techniques), xeno-free supplement designed for hMSC culture. Manufactured under GMP and ISO 13485 certified factory, with US FDA Master File Number.

Primary culture of hMSCs using AllPhase shows superior efficiency. Excellent expansion rate (doubling time under 30 hours) of either UC-MSC, ADSC or BM-MSC was observed.

The ability of self-renewal and multi-lineage differentiation of MSCs remained for several passages.

Data

Data

Superior primary culture performance

AllPhase primary culture comparison

Total number of cells isolated from 6 cm sections of umbilical cord. Tissue sections were minced and left undisturbed for 18 days on two 100 mm TC-treated or CellBIND culture plates. Medium was replaced every 3 days. AllPhase demonstrates superior primary culture performance.

MSC morphology from P0 to P5

P0

AllPhase morphology P0

P1

AllPhase morphology P1

P3

AllPhase morphology P3

P5

AllPhase morphology P5

Using AllPhase for primary culture of human MSC. Normal morphology was observed; cells retain spindle shape and remain healthy from P0 to P5.

Immunophenotype of MSC by flow cytometry

P1

AllPhase surface markers P1

P3

AllPhase surface markers P3

Flow cytometry analysis of human MSC cultured in AllPhase. Cells were positive for CD90, CD105, CD73 and CD44.

Trilineage differentiation ability

Osteogenesis
Adipogenesis
Chondrogenesis
Control
AllPhase diff Control Osteogenesis
AllPhase diff Control Adipogenesis
AllPhase diff Control Chondrogenesis
Differentiated
AllPhase diff Differentiated Osteogenesis
AllPhase diff Differentiated Adipogenesis
AllPhase diff Differentiated Chondrogenesis

After culturing in AllPhase, human MSC demonstrates the ability to differentiate into osteocyte, adipocyte and chondrocyte.

Custom Services

CMO / CDMO Custom Manufacturing Services

All culture media products are available for CMO (Contract Manufacturing) and CDMO (Contract Development and Manufacturing) services, including formula customisation, specification tailoring, regulatory documentation, and full-scale support from small batches to mass production.

Documents

User manual and more information are available upon request.